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viability dye efluor780  (Thermo Fisher)


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    Structured Review

    Thermo Fisher viability dye efluor780
    Viability Dye Efluor780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viability+dye+efluor780/PBS/pmc12664390-341-31-34
    Average 99 stars, based on 1 article reviews
    viability dye efluor780 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    FACS:

    Article Title: A versatile mouse model to advance human microglia transplantation research in neurodegenerative diseases.
    Article Snippet: The remaining cell pellet was washed with FACS buffer (2% FBS (Life Technologies, 10,270,106)), 2 mM EDTA (Sigma-Aldrich, E7889) in PBS) and blocked with FcR blocking solution (1:10, Miltenyi, 130–092–575 & 130– 059–901) for 10 min at 4 °C. .. One-fourth of the sample was used for FACS analysis (see below) and the remainder was stained with viability dye eFluor780 (1:1000, Thermo Fisher, 65–0865-14) and antibodies for 30 min at 4 °C: anti-CD11b (1:50, Milteny, 130–113–806), antihCD45 (1:50, BD Bioscience, 555,485), anti-mCD45 (1:200, BD Bioscience, 563,890). .. Samples were run on the BD FACSCantoTM flow cytometer or Miltenyi MACS Quant Tyto cell sorter.

    Article Title: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
    Article Snippet: .. For fluorescently activated cell sorting (FACs), cells from digested hydrogels were first filtered through a 70 μm cell strainer (Falcon) and the extracellular staining was performed as previously described while the viability dye efluor780 (eBioscience) was used (1:1000 in PBS). ..

    Article Title: A versatile mouse model to advance human microglia transplantation research in neurodegenerative diseases
    Article Snippet: The remaining cell pellet was washed with FACS buffer (2% FBS (Life Technologies, 10,270,106)), 2 mM EDTA (Sigma-Aldrich, E7889) in PBS) and blocked with FcR blocking solution (1:10, Miltenyi, 130–092–575 & 130–059–901) for 10 min at 4 °C. .. One-fourth of the sample was used for FACS analysis (see below) and the remainder was stained with viability dye eFluor780 (1:1000, Thermo Fisher, 65–0865-14) and antibodies for 30 min at 4 °C: anti-CD11b (1:50, Milteny, 130–113–806), anti-hCD45 (1:50, BD Bioscience, 555,485), anti-mCD45 (1:200, BD Bioscience, 563,890). .. Samples were run on the BD FACSCantoTM flow cytometer or Miltenyi MACS Quant Tyto cell sorter.

    Article Title: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
    Article Snippet: .. For fluorescently activated cell sorting (FACs), cells from digested hydrogels were first filtered through a 70 μm cell strainer (Falcon) and the extracellular staining was performed as previously described while the viability dye efluor780 (eBioscience) was used (1:1000 in PBS). ..

    Staining:

    Article Title: A versatile mouse model to advance human microglia transplantation research in neurodegenerative diseases.
    Article Snippet: The remaining cell pellet was washed with FACS buffer (2% FBS (Life Technologies, 10,270,106)), 2 mM EDTA (Sigma-Aldrich, E7889) in PBS) and blocked with FcR blocking solution (1:10, Miltenyi, 130–092–575 & 130– 059–901) for 10 min at 4 °C. .. One-fourth of the sample was used for FACS analysis (see below) and the remainder was stained with viability dye eFluor780 (1:1000, Thermo Fisher, 65–0865-14) and antibodies for 30 min at 4 °C: anti-CD11b (1:50, Milteny, 130–113–806), antihCD45 (1:50, BD Bioscience, 555,485), anti-mCD45 (1:200, BD Bioscience, 563,890). .. Samples were run on the BD FACSCantoTM flow cytometer or Miltenyi MACS Quant Tyto cell sorter.

    Article Title: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
    Article Snippet: .. For fluorescently activated cell sorting (FACs), cells from digested hydrogels were first filtered through a 70 μm cell strainer (Falcon) and the extracellular staining was performed as previously described while the viability dye efluor780 (eBioscience) was used (1:1000 in PBS). ..

    Article Title: Expression of human CEACAMs promotes inflammation and organ damage during systemic Candida albicans infection in mice
    Article Snippet: .. Cells were stained using viability dye eFluor780 (eBiosciences), anti-CD11b-APC (clone REA592, Miltenyi Biotec), and either 308/3-3 (anti-human CEACAM3/5, LeukoCom, Essen, Germany) / highly cross-adsorbed goat anti-mouse IgG1-PE (eBiosciences), or 1H7-4B (anti-human CEACAM6, LeukoCom, Essen, Germany) / highly cross-adsorbed goat anti-mouse IgG1-PE (eBiosciences), respectively. ..

    Article Title: A versatile mouse model to advance human microglia transplantation research in neurodegenerative diseases
    Article Snippet: The remaining cell pellet was washed with FACS buffer (2% FBS (Life Technologies, 10270106)), 2 mM EDTA (Sigma-Aldrich, E7889) in PBS) and blocked with FcR blocking solution (1:10, Miltenyi, 130-092-575) for 10min at 4°C. .. As a last step, cells were stained with viability dye eFluor780 (1:1000, Thermo Fisher, 65- 0865-14) and antibodies for 30min at 4°C: anti-CD11b (1:50, Milteny, 130-113-806), anti-hCD45 (1:50, BD Bioscience, 555485), anti-mCD45 (1:200, BD Bioscience, 563890). .. Samples were run on the BD FACSCanto TM flow cytometer or Miltenyi MACS Quant Tyto cell sorter.

    Article Title: A versatile mouse model to advance human microglia transplantation research in neurodegenerative diseases
    Article Snippet: The remaining cell pellet was washed with FACS buffer (2% FBS (Life Technologies, 10,270,106)), 2 mM EDTA (Sigma-Aldrich, E7889) in PBS) and blocked with FcR blocking solution (1:10, Miltenyi, 130–092–575 & 130–059–901) for 10 min at 4 °C. .. One-fourth of the sample was used for FACS analysis (see below) and the remainder was stained with viability dye eFluor780 (1:1000, Thermo Fisher, 65–0865-14) and antibodies for 30 min at 4 °C: anti-CD11b (1:50, Milteny, 130–113–806), anti-hCD45 (1:50, BD Bioscience, 555,485), anti-mCD45 (1:200, BD Bioscience, 563,890). .. Samples were run on the BD FACSCantoTM flow cytometer or Miltenyi MACS Quant Tyto cell sorter.

    Article Title: CD8+ T cells drive myofibroblast activation and contraction via JAK/STAT3 and TGFβ signaling
    Article Snippet: .. For fluorescently activated cell sorting (FACs), cells from digested hydrogels were first filtered through a 70 μm cell strainer (Falcon) and the extracellular staining was performed as previously described while the viability dye efluor780 (eBioscience) was used (1:1000 in PBS). ..

    Flow Cytometry:

    Article Title: A vaccine platform targeting lung-resident memory CD4 + T-cells provides protection against heterosubtypic influenza infections in mice and ferrets
    Article Snippet: .. Viability dye eFluor780 - Flow cytometry eBioscience 65-0865-14 mCD11b PE/Cyanine7 M1/70 Flow cytometry (For innate cells) BioLegend 101216 mCD11c APC N418 BioLegend 117310 mLy6G PerCP/Cyanine5.5 1A8 BioLegend 127616 mF4/80 PE BM8 BioLegend 123110 mCD40 FITC 3/23 Flow cytometry (For DC activation) BioLegend 124607 mCD80 PE 16-10A1 BioLegend 104708 mCD86 PE/Cyanine5 GL-1 BioLegend 105016 mMHC-II PE/Cyanine7 AF6-120.1 BioLegend 116420 mCD4 PE/Cyanine7 RM4-5 Flow cytometry (For T-cell analysis) BioLegend 100528 mCD8a FITC 53-6.7 BioLegend 100706 mCD44 APC IM7 BioLegend 103011 mCD62L PE/Cyanine5 MEL-14 BioLegend 104410 mCD69 PE H1.2F3 BioLegend 104507 mCD103 PerCP/Cyanine5.5 2E7 BioLegend 121415 mIFN-γ PE XMG1.2 BioLegend 505808 mIL-4 PerCP/Cyanine5.5 11B11 BioLegend 504123 mIL-17A eFlour 660 eBio17B7 Invitrogen 50-7177-82 mIgG-HRP - - ELISA SouthernBiotech 1030-05 mIgG1-HRP - - SouthernBiotech 1070-05 mIgG2a-HRP - - SouthernBiotech 1080-05 mIgA-HRP - - SouthernBiotech 1040-05 Supplementary Table 2. ..

    Activation Assay:

    Article Title: A vaccine platform targeting lung-resident memory CD4 + T-cells provides protection against heterosubtypic influenza infections in mice and ferrets
    Article Snippet: .. Viability dye eFluor780 - Flow cytometry eBioscience 65-0865-14 mCD11b PE/Cyanine7 M1/70 Flow cytometry (For innate cells) BioLegend 101216 mCD11c APC N418 BioLegend 117310 mLy6G PerCP/Cyanine5.5 1A8 BioLegend 127616 mF4/80 PE BM8 BioLegend 123110 mCD40 FITC 3/23 Flow cytometry (For DC activation) BioLegend 124607 mCD80 PE 16-10A1 BioLegend 104708 mCD86 PE/Cyanine5 GL-1 BioLegend 105016 mMHC-II PE/Cyanine7 AF6-120.1 BioLegend 116420 mCD4 PE/Cyanine7 RM4-5 Flow cytometry (For T-cell analysis) BioLegend 100528 mCD8a FITC 53-6.7 BioLegend 100706 mCD44 APC IM7 BioLegend 103011 mCD62L PE/Cyanine5 MEL-14 BioLegend 104410 mCD69 PE H1.2F3 BioLegend 104507 mCD103 PerCP/Cyanine5.5 2E7 BioLegend 121415 mIFN-γ PE XMG1.2 BioLegend 505808 mIL-4 PerCP/Cyanine5.5 11B11 BioLegend 504123 mIL-17A eFlour 660 eBio17B7 Invitrogen 50-7177-82 mIgG-HRP - - ELISA SouthernBiotech 1030-05 mIgG1-HRP - - SouthernBiotech 1070-05 mIgG2a-HRP - - SouthernBiotech 1080-05 mIgA-HRP - - SouthernBiotech 1040-05 Supplementary Table 2. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A vaccine platform targeting lung-resident memory CD4 + T-cells provides protection against heterosubtypic influenza infections in mice and ferrets
    Article Snippet: .. Viability dye eFluor780 - Flow cytometry eBioscience 65-0865-14 mCD11b PE/Cyanine7 M1/70 Flow cytometry (For innate cells) BioLegend 101216 mCD11c APC N418 BioLegend 117310 mLy6G PerCP/Cyanine5.5 1A8 BioLegend 127616 mF4/80 PE BM8 BioLegend 123110 mCD40 FITC 3/23 Flow cytometry (For DC activation) BioLegend 124607 mCD80 PE 16-10A1 BioLegend 104708 mCD86 PE/Cyanine5 GL-1 BioLegend 105016 mMHC-II PE/Cyanine7 AF6-120.1 BioLegend 116420 mCD4 PE/Cyanine7 RM4-5 Flow cytometry (For T-cell analysis) BioLegend 100528 mCD8a FITC 53-6.7 BioLegend 100706 mCD44 APC IM7 BioLegend 103011 mCD62L PE/Cyanine5 MEL-14 BioLegend 104410 mCD69 PE H1.2F3 BioLegend 104507 mCD103 PerCP/Cyanine5.5 2E7 BioLegend 121415 mIFN-γ PE XMG1.2 BioLegend 505808 mIL-4 PerCP/Cyanine5.5 11B11 BioLegend 504123 mIL-17A eFlour 660 eBio17B7 Invitrogen 50-7177-82 mIgG-HRP - - ELISA SouthernBiotech 1030-05 mIgG1-HRP - - SouthernBiotech 1070-05 mIgG2a-HRP - - SouthernBiotech 1080-05 mIgA-HRP - - SouthernBiotech 1040-05 Supplementary Table 2. ..



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